Technology

TissueFAXS SPECTRA is based on a liquid crystal tunable filter and the spectral unmixing engine of StrataQuest analysis software. Liquid crystal tunable filters are optical filters that use electronically controlled liquid crystal elements to transmit a selectable wavelength of light and exclude others. They can be tuned to specific wavelengths within the visible spectrum at high speeds. This makes them ideal for a quick building of the lambda stacks (a.k.a “Image Cubes” or “Spectral Cubes”), which then can be used for spectral unmixing.

This permits the separation of multiple colors in both, brightfield and fluorescence. The following graphic shows the process from building the lambda stack to color deconvolution via the StrataQuest spectral unmixing engine. The result is four greyscale channel images corresponding to the four unmixed colours and an overlay image analogous to a fluorescence overlay image.