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Video Tutorials

P-2000 Introduction
Pulling a Patch Pipette From Quartz Glass
Effects of The Velocity Parameter
Copying Pull Programs on the P-2000 Puller
Troubleshooting Pipette Variablity (video)
Puller Bars and V-groove Bearings (video)
Checking the Pull Cables (video)
Checking the Cable Pulleys (video)

While the Sutter P-2000 is suitable for working with most conventional glasses, its primary advantage is the ability to work with quartz glass (fused silica). Quartz offers superior material properties for a variety of research applications. Quartz is stronger than other glasses and can facilitate penetration through tough tissues which would normally break conventional pipettes . For applications requiring a low noise glass, users will find that quartz is the lowest noise glass available. Quartz contains none of the metals used in conventional glasses. Optically, quartz is virtually free from fluorescence when illuminated.

A CO₂ laser was selected as the heat source for the P-2000 for several reasons. First, the nominal emission wavelength of the laser approximates the resonant frequency of the SiO₂ lattice in glass. Thus, quartz and other conventional glasses can be melted when the appropriate laser power is supplied. Second, laser heat is clean and leaves no metal residue on the pipette as do conventional heating filaments. Third, laser heat can be turned off instantly, leaving no residual filament heat. Lastly, the user can program the amount and distribution of heat supplied to the glass.

The P-2000 can store up to 100 separate programs, with each program consisting of up to 8 command lines. Programmable parameters include; laser power level, scan width, trip velocity, delay/ laser on time, and hard pull strength.

One important consideration for the use of the Sutter P-2000 is the diameter of the glass used. The Sutter P-2000/G is designed to produce even heating on glass up to 1.2 mm in outside diameter. Larger diameter glasses can be used with the Sutter P-2000/G (up to 1.5 mm quartz and 1.8 mm conventional glasses), but the performance is best with glass that is 1.2 mm diameter or less.

The Sutter P-2000/F works well with small diameter glasses such as optical fibers, and with small diameter fused silica capillary commonly used for the manufacture of nanospray tips. Small diameter glass (outer diameter in the range of 0.125 mm to 0.6 mm) requires special puller bars as well as an optical alignment optimized for the smaller diameter material.

As with larger diameter glass, a wide range of tip sizes and taper geometries can be produced with the Sutter P-2000/F and small diameter glass. We have drawn optical fiber tips ranging from less than 10nm to more than 5µm. Please consult our technical staff for further information.

The P-2000 micropipette puller represents a significant advance in the technology of fabrication of micropipettes, optical fiber probes, and nanospray tips. The P-2000 integrates a CO₂ laser-based heat source with the technology derived from our extensive experience with conventional pullers. This system offers capabilities unmatched by other pullers.

Features

• Capable of pulling quartz, borosilicate and aluminosilicate glass.
• Fully programmable – including heating filament characteristics.
• The laser has no melting point limit as with conventional metal filaments; and therefore, cannot be burned out.
• Pulls electrodes with tip diameters smaller than 0.03µm.
• Optimized velocity sensing circuit for maximized sensitivity and reproducibility.*
• Operating life of the CO₂ laser is expected to be in excess of ten years with normal use, after which the laser can be refurbished by the Sutter Instrument Company for a nominal charge.
• Individual programs can be write-protected in order to secure them from inadvertent changes.
• The total time that the heat is on during the pull is displayed for improved program development and troubleshooting.
• A date and time stamp is displayed to show the last time that a program has been changed.
• The P-2000/F is ideal for applications such as nanospray and NSOM.
• Preprogrammed sample programs for intra-cellular and patch pipettes. The P-2000/F also comes with an NSOM tip program.