Nightsea Fluorescence Adapter for Keyence VHX-5000

Fluorescent mineral sample imaged with the Keyence + NIGHTSEA systems

Fluorescence Adapter for Keyence VHX-5000

The NIGHTSEA Model SFA Fluorescence Adapter system adds fluorescence to the Keyence VHX-5000 Series Digital Microscope using the same approach that we take with conventional stereo microscopes – the combination of an LED-based light source with a matching barrier filter. The system consists of a free-standing lamp that accepts modular LED light heads, a barrier filter mounted in a holder that slips over the end of the Keyence lens*, and a pair of barrier filter glasses.

*Fits on the VH-Z00, VH-Z20, VH-Z50x and VH-Z100 series lenses

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Five excitation light heads and matching barrier filters (glasses not shown)

Detail of light head, and barrier filter fitted over the Keyence lens

Fluorescent-dosed plastic particles, 38 – 45 microns. Image made with ultraviolet excitation. Image courtesy of an explosives detection company.

Wavelengths

There are five excitation/emission combinations available for the system.

Ultraviolet (UV) – Ex – 360 – 380nm, Em – 415nm longpass
Violet (VI) – Ex – 400 – 415nm, Em – 460nm longpass
Royal Blue (RB) – Ex – 440 – 460nm, Em – 500 nm longpass or 500 – 560nm bandpass
Cyan (CY) – Ex – 490 – 515nm, Em – 550nm longpass
Green (GR) – Ex – 510 – 540nm, Em – 600nm longpass

Part numbers and pricing

One-color complete setup – $1,095 ($1,245 for UV)
- SFA-K-UV – Ultraviolet
- SFA-K-VI – Violet
- SFA-K-RB – Royal Blue with longpass filter
- SFA-K-RB-GO – Royal Blue with green bandpass filter
- SFA-K-CY – Cyan
- SFA-K-GR – Green
Modular excitation/emission sets – $555 ($705 for UV)
- SFA-K-LFS-UV – Ultraviolet
- SFA-K-LFS-VI – Violet
- SFA-K-LFS-RB – Royal Blue with longpass filter
- SFA-K-LFS-RB-GO – Royal Blue with green bandpass filter
- SFA-K-LFS-CY – Cyan
- SFA-K-LFS-GR – Green