Objective Imaging Mosaic Imaging Software

Surveyor Software with Turboscan, for Real-Time Image Stitching in X, Y, and Z

Objective Imaging Surveyor software with Turboscan enables scanning and near perfect-image stitching at camera frame rates. Scan the entire slide in seconds, and then effortlessly relocate to areas of interest with a simple click. Quickly get the specimen overview to aid in understanding the relationships between microscopic features and overall structure. Save your work for later review, discussion, and publication. Based on the OASIS-blue controller with the BLUE-EXPIO Encoder and Trigger interface module or OASIS-AF analog video processor option, Surveyor software with Turboscan provides everything a microscopist needs for surveying, relocating, printing and saving at a new standard in high-performance automation.


 

OASIS Software

 

OASIS Software

 

OASIS Software

 

 

OASIS Software

Slide Overview and Image Stitching

The demand for converting microscopic slides into virtual slides is growing. Long-term stability and easy sharing are the reasons why many labs, appreciate the convenience of virtual slides. The slide scanning platform using Objective Imaging’s Surveyor software with TurboScan and a Leica automated microscope equipped with a high precision Märzhäuser encoded motorized stage allows for fast digitization of highest quality images.

 

Virtual slides from various staining techniques
Being a microscope based system; Surveyor supports a wide range of contrasting techniques, including fluorescence as well as images composed of more than three color channels.  Even mixing of contrasting modes in the same virtual slide is supported.

 

Multiple region of interest
Sample tissue sections can be pre-scanned at low power and multiple regions of interest can be quickly captured for storage and review.

 

Fastest imaging scanning of any microscope based instrument
TurboScan offers two working modes: images can be processed during the scan, so that the final virtual slide is available immediately after the system has acquired the last tile. Alternatively, the single images can be stored and processed later, even on another system or with other parameters. This way it is easy to generate partial images from the same set of data, using only selected color channels.

 

Whether your goal is seamless integration into a flagship automated imaging system, or customized operation for a unique application, Objective Imaging's complete range of automation control software ensures you are fully utilizing the OASIS line of automation control products with minimal effort.

 

Surveyor with Turboscan Highlights:

  • Scanning and acquisition at camera frame rates, as fast as 100 micro-seconds
  • Support for various digital cameras, including QImaging QICAM and Leica Microsystems DFC camera series
  • High-quality image tiling
  • Frame-rate shading correction
  • Fast and accurate relocation
  • Continuous focus tracking using predictive focus
  • Compatible with most 3rd party motorized stage and focus drives
  • Intelligent memory management supports mosaic sizes limited only by available disk space
  • Available as stand-alone application and as a capture driver for use within Image-Pro® Plus

 

Ultra-fast microscope image stitching in any illumination technique including fluorescence enhances all research imaging applications. View large assembled images in 3D with optional 3D topography software.

 
Ultra-fast microscope image stitching in any illumination technique including fluorescence enhances any research imaging application. View large assembled images in 3D with optional 3D topography software. Ultra-fast microscope image stitching in any illumination technique including fluorescence enhances any research imaging application. View large assembled images in 3D with optional 3D topography software.

Turboscan Examples:

Click on the images above for a larger view

 

Turboscan Zoomify Examples:
zoomify.com


Mouse captured at 5x magnification


285 tiles X-Y dimension of 25,720 x 14,792 pixels, 1.14 GB file, 2.45 minutes to scan


Mouse brain captured at 10x magnification


162 tiles X-Y dimension of 17,585 x 10,889 pixels, 574 MB file, 2.3 minutes to scan

Click on the images for a Zoomify version

Turboscan Examples Using Zoomify:

Click on the images above for a larger view

 


Surveyor with Turboscan software uses the Märzhäuser SCAN for upright microscopes and SCAN IM for inverted microscopes stages

Märzhäuser SCAN for upright microscopes

 

Märzhäuser SCAN IM for inverted microscopes

 

Märzhäuser SCAN-Series Motorized Microscope Stages

The SCAN series has been developed especially for applications that require automatic and very exact positioning of samples. Every stage comes with encoders.

 

A special long-term lubrication system guarantees that specifications are maintained, even after a very long running period.

 

The SCAN series motorized microscope stages are simply the best available today.

 

The SCAN series are used exclusively with Surveyor and Turboscan software.


 


Downloads:


Objective Surveyor Software with Turboscan for Real-Time Mosaic Imaging Brochure Objective Surveyor Software with Turboscan for Real-Time Mosaic Imaging Brochure
Leica DM6000 B Research Microscope Brochure Leica DM6000 B Research Microscope Brochure
Leica DMI6000 B Inverted Microscope Brochure Leica DMI6000 B Inverted Microscope Brochure

 

 

 

 

Flurorescent Brain Section Scanned with a 10x Objective in X, Y, and Z with Extended Depth of Field, using Surveyor with Turboscan software

Click on the image above for a large view

 

Flurorescent Brain Section Scanned with a 10x Objective in X, Y, and Z with Extended Depth of Field, using Surveyor with Turboscan software. Turboscan mosaic of over 340 images, digital camera resolution: 1340 x 1022 pixels, scanned with predictive focus, shading correction, and extended depth of field, total time for scan and mosaic: 192 seconds.


Books to Consider Reading


The Image Processing Handbook, 4th Edition Digital Image Processing : Principles and Applications Unbiased Stereology: Three-Dimensional Measurement in Microscopy Optimizing Light Microscopy for Biological and Clinical Laboratories

The Image Processing Handbook, 4th Edition


by Dr. John C. Russ (2002)

Digital Image Processing : Principles and Applications


by Gregory A. Baxes (1994)

Unbiased Stereology: Three-Dimensional Measurement in Microscopy

 

by C.V. Howard and M.G. Reed (1998)

Optimizing Light Microscopy for Biological and Clinical Laboratories

 

by Barbara Foster (1997)

Practical Stereology, Second Edition Video Microscopy Video Microscopy: The Fundamentals, 2nd Edition Electronic Failure Analysis Handbook : Techniques and Applications for Electronic and Electrical Packages, Components, and Assemblies

Practical Stereology, Second Edition

 

by John C. Russ and Robert T. Dehoff (2000)

Video Microscopy

 

Edited by Greenfield Sluder and David E. Wolf (1998)

Video Microscopy: The Fundamentals, 2nd Edition


by Shinya Inoue' and Kenneth R. Spring (1997)

Electronic Failure Analysis Handbook : Techniques and Applications for Electronic and Electrical Packages, Components, and Assemblies

 

by Perry L. Martin (February 1999)

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