In-Focus Automation

Traditional Imaging
In-Focus Automation
In-Focus and Extended Depth of Field
Traditional Imaging
In-Focus Automation
In-Focus and Extended Depth of Field

In-Focus Automation solves a 150-year imaging problem by finally being able to provide scientists and researchers the image they could only imagine, namely one that is completely in focus. The narrow depth of field associated with optical microscopy has always required microscopists to continually focus back and forth through the z-axis of an object. If a picture was required, even selecting the "best picture" still left most of the image out of focus. Now, one can effortlessly acquire a series of multi-focused images and within seconds produce an Extended Depth of Field (EDF) picture. More than 100-times the original depth of field is possible!

 

In-Focus Automation was designed to eliminate narrow depth of field problems associated with reflected or transmitted light microscopy. Any optical technique can be used. In-Focus Automation controls your motorized stereomicroscope or macroscope and camera system to produce a "sequence" or "stack" of images. Each image is taken at different focus points within the subject. In-Focus Automation then uses Image-Pro Plus’ Extended Depth of Field (EDF) function to combine the multiple images in the sequence into a single "In-Focus" image. The composite image can reveal a great deal of information within the sample that may otherwise be obscured or unnoticed.

 

In-Focus Automation is a simple to use program operating with Image-Pro Plus extended depth of field function. It can be used with any properly configured Leica MZ motorized stereomicroscope or motorized macroscope. In-Focus Automation works best with digital cameras but it can also operate with analog video cameras and Image-Pro Plus supported frame grabbers. A complete list of supported products is available on request. Let us show you the difference In-Focus Automation makes!


In-Focus Automation Example Images

(click on the Flash icon to view an animation)

Traditional Imaging
In-Focus & Extended Depth of Field
Extended Depth of Field
Ant
Extended Depth of Field
Metal Fatigue
Extended Depth of Field
Mouse Eye
Extended Depth of Field
Foram
Extended Depth of Field
Moth Head
Extended Depth of Field
Mouse Skull
Extended Depth of Field
Pupa
Extended Depth of Field
Fly Muscle DIC
Extended Depth of Field
Fly
Extended Depth of Field
Fungus
Extended Depth of Field
Mitosis
Extended Depth of Field
Electronic Chip
Extended Depth of Field
Frog Egg
Extended Depth of Field
Flint

Books to Consider Reading


       
The Image Processing Handbook, 4th Edition Digital Image Processing : Principles and Applications Unbiased Stereology: Three-Dimensional Measurement in Microscopy Optimizing Light Microscopy for Biological and Clinical Laboratories

The Image Processing Handbook, 4th Edition


by Dr. John C. Russ (2002)

Digital Image Processing : Principles and Applications


by Gregory A. Baxes (1994)

Unbiased Stereology: Three-Dimensional Measurement in Microscopy

 

by C.V. Howard and M.G. Reed (1998)

Optimizing Light Microscopy for Biological and Clinical Laboratories

 

by Barbara Foster (1997)

Practical Stereology, Second Edition Video Microscopy Video Microscopy: The Fundamentals, 2nd Edition Electronic Failure Analysis Handbook : Techniques and Applications for Electronic and Electrical Packages, Components, and Assemblies

Practical Stereology, Second Edition

 

by John C. Russ and Robert T. Dehoff (2000)

Video Microscopy

 

Edited by Greenfield Sluder and David E. Wolf (1998)

Video Microscopy: The Fundamentals, 2nd Edition


by Shinya Inoue' and Kenneth R. Spring (1997)

Electronic Failure Analysis Handbook : Techniques and Applications for Electronic and Electrical Packages, Components, and Assemblies

 

by Perry L. Martin (February 1999)

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